癫痫患者血清、血清超滤液、唾液、脑脊液中苯妥英浓度的相关性和动力学研究

Study on the kinetics and correlation of the contents of phenytion in the serum serum ultrafiltrate,saliva and cerebrospinal fluid in patients with epilepsy

本文应用超滤法测定血清中游离苯妥英浓度的方法,并采用荧光酶免疫法对患者的血清、血清超滤液、唾液、脑脊液进行苯妥英浓度测定,研究它们的相关性。通过测定6名癫痫患者单剂量口服苯妥英钠后血清、血清超滤液、唾液中的药物浓度,计算其动力学参数。实验结果表明,血清超滤液、唾液、脑脊液中苯妥英浓度分别为血清药物浓度的11%、10%、11%,有良好的相关性。各样品测得的动力学数据基本一致,药物浓度—时间曲线变化相同。血清超滤液、唾液、脑脊液样品中苯妥英浓度均反映了患者体内游离药物浓度的情况,唾液样品收集方便、迅速,对患者无损伤,适用于临床进行治疗药物监测。

In the present experiment an ultrafiltring method was employed to determine the concentration of unbound drugs in theserum.The contents of phenytion in the serum,serum ultrafiltrate,saliva and cerebrospinal fluid in 6 patients with epilepsyafter oral administration of the drug in a single dose were measured using fluorecence enzyme immunoassay and the kineticparameters ot phengtion were calculated from the experimental data.The findings indicated that there was a significantcorrelation in the contents of phengtion of various samples and the contents of phengtion in the serum ultrafiltrate,saliva andcerebrospinal fluid were 11%,10% and 11% that in the serum.The half-life and peak time of the various samples wereroughly in concordance and changes in concentration-time curve were identical the contents of drugs in the serum ultrafiltrate,saliva anti cerebrospinal fluid indicated the in vivo status of unbound drugs.Saliva sample might be used in monitoring therapybecause it is readily collectable,rapid and non-invasive.