石墨炉原子吸收光谱法测定糖尿病患者血清中微量锰和铬

The determination of trace Mn and Cr in serum of patients with glycosuria disease by graphite furnace atomic absorption spectrometry

本文采用石墨炉原子吸收光谱法测定糖尿病患者血清中微量锰和铬。测定条件:锰波长为279.5nm,狭缝1.9(?),灯电流6mA,干燥150℃/30s,灰化1000℃/20s,原子化2200℃/4s。铬波长为357.9nm,狭缝3.8(?),灯电流6mA,干燥150℃/30s,灰化500℃/20s,原子化2500℃/4s。方法的回收率、灵敏度和CV%:锰为105.0%,0.77PPb,6.5%。铬为96.8%,0.30ppb,4.5%。

The trace Mn and Cr in serum of patients with glycosuria disease was determined by graphite furnace atomic absorptionspectrometry.Conditions for Mn:wavelength 179.5nm,slit width 1.9A,light electric current 6mA,drying step 150℃/30s,ashing step 1000℃/20s,atomic step 2200℃/4s;conditions for Cr:wavelength 257.9nm,slit width 3.8(?),light electriccurrent 6mA,drying step 150℃/30s,ashing step 500℃/20s,atomic step 2500℃/4s.The recovery,sensitivity and RSD were105.0%,0.77ppm,6.5% for Mn;96.8%,0.30ppm,4.5% for Cr.