高效液相色谱法测定人血清中氨吡酮浓度

Determination of amrinone in serum by HPLC

本文建立了测定人血清中氨吡酮浓度的HPLC 方法学,具有方法简便、取样量小、灵敏度高、专一性强、回收稳定等优点,并进行了临床病人的血药浓度监测,方法可靠。

A method,of determination of amrinone in serum by HPLC had been studied.The eluent was phosphate buffer:methanol:acetonitrile=380:80:40.The serum samples were deproteinizied by adding acetonitrile.The upper layer was evaporatedto dryness at 40℃ under a gentle stream air.The residue were reconstituted in 200μl mobile phase of which 50μl were in-jected onto a C_(18) column.The detection limit was 0.267 nmol.The average recovery was 99.2±2.9%.T_(1/2):9.74h;V:13.77L.There were 24 serum samples of clinical patients were monitored.The average serum concentration was 4.29±3.7μmol/L.It sugguested when a usually regimen amrinone was used,the vasodilatation was the main action to cure cardiac fail-ure.If raising amrinone dose properly and matching TDM,there will be more greatly curative effect.