抗癌中药制剂局部注射对裸鼠人肝癌细胞核 DNA 含量的影响

Change of the Nuclear DNA Content after Local Injectin of Anti-cancer Chinese Medicine in Transplanted Human Hepatocellular Carcinoma in Nude Mice

应用自动化图像分析技术对裸鼠人肝癌经局部注射抗癌中药制剂治疗的肝癌细胞核DNA含量进行定量测定,结果表明:经局部治疗后的DNA相时含量均下降(由22.57降到16.13,15.86),与对照组间盖异有显著意义(P<0.01),病理组织学改变为癌组织坏死,纤维组织增生及炎细胞浸润。因此作者认为,洲定DNA含量和组织病理学观察,对抗癌药物疗效的评价,具有重要意义。

This paper reports a morphometric study on the nuclear DNA content of transplanted human hepatocellular carcinoma in 19 nude mice after local injection of anti-cancer chinese medicine using automatic image analysis instrument. The results showed that the average content of nuclear DNA in treated group (16.13fu) was reduced as compared with the control group (22.57fu P<0. 01). Necrosis of tumor cells, proliferation of fibroblasts, infiltration of inflammatory cells were found microscopically in the tumor tissues of the treated group. It is suggested that the measurement of nuclear DNA content and morphology are of help in assessing results after treatment with anti-cancer chinese medicine in cancers.