半乳糖氧化酶试验检测大肠癌

Detection of Large Bowel Cancer Using Galactose Oxidase Test

用半乳糖氧化酶-雪夫液方法检测35例结直肠癌及癌前病变组织切片中的β-D-GalGalNAc,94.28n/o阳性。制成试纸检测924例肛检粘液涂片。3.14％阳性。12例结直肠癌及5例癌前病变(腺瘤3,异型增生2)呈阳性,敏感性为91.14％(17/18),阳性预检值58.62％(17/29)。有结直肠症状组阳性率显著高于无症状组(P<O.001。该试验方便快速,可望用于大肠癌门诊初筛与高危人群普查。

β-D-GalGalNAc was determined in the specimens from 35 cases of the colorectal carcinoma, ade- noma and the atypical heperplasia using galactose oxidase sequence (Go-S). The positive rate was 94. 28%. With the test paper saturated with Go, the digital mucous smears from 924 cases were tested and the positive rate was3. 14%. Symp- tomatic group had a higher positivity than asymptomatic group (P<0. 001 ). Seventeen of 18 Datients with colorectal can- cer and precancerous disease were positive (sensitivity 94. 44% and positive predictive value 58. 62%). The results showed that Go-S rest is warranted in mass screening for large bowel neoplasms.