凝集素在研究复发性肝癌细胞克隆来源中的可能价值

Potential Significance of Lectins in the Study of Cellular Clone Origin of Recurrent Hepatocellular Carcinoma

用ABC法对16例二次切除的肝细胞癌(HCC)组织中凝集素受体进行了测定,并观察了裸鼠体内传代2年多的人HCC组织中凝集素受体的变化,同时对这些肝癌病人的肿瘤部位、复发时间、肿瘤的某些生物学特性进行了统计学分析。结果表明:裸鼠体内传代的人HCC组织中凝集素受体变化很小。一些二次切除的HCC之间凝集素受体差别较少,而另一些则差别较多。这种二次切除的肝癌组织间凝集素受体的差别与肿瘤的部位、复发时间和某些生物学特性有明显的关系。提示:与原发肝癌相比,凝集素受体差别较多的复发性肝癌可能来自于不同的肿瘤细胞克隆;凝集素在探讨复发性肝癌细胞克隆来源中有潜在的应用价值。

Lectin receptors were determined in 16 cases of resected recurrent hepatocellular carcinoma (HCC) with avidin biotin-peroxidase complex (ABC)method. Changes of the lectin receptors in human HCC explants in nude mice after propagation for more than 2 years were observed. Tumor location. recurrence time and some of their biological characteristics were statistically analysed in patients. The results showed that lectin receptors of human HCC explants in nude mice showed ncgligible changes in a rather long period of time. Leetin receptors in resected recurrent HCC varied from case to case. These differences were obviousty related to tumor location, cecurrence time as well as certain character- isties of tumor in patients. It is suggested that recurrent HCC which showed dissimilar contents of lectin receptors from its primary HCC might have originated from different cancer cell clone. Lectins have potential value if applied in the study of the cell clone origin of recurrent tumor.