冲击波对兔角膜内皮细胞损伤及其修复的实验研究

An Experimental Study on Injury and Recovery of Rabbit Corneal Endothelial Cells after Blast

冲击波致伤原代培养兔角膜内皮细胞后,于伤后不同时间用光镜、扫描电镜和透射电镜观察细胞形态与结构的改变,测定细胞Na/K ATP酶的活性,由此反映细胞层损伤及其修复,并将此过程分为三期:①急性损伤期:细胞脱落、变性、坏死,Na/K ATP酶活性降低;②再生期:存活细胞移行、分裂增生,Na/K ATP酶活性缓慢回升;③恢复期:大部存活细胞恢复多边形。Na/K ATP酶活性上升显著。初步探讨了冲击波损伤细胞的致伤机理。

After blast injury, the morphologie and ultrastructural differentiation of the rabbit corneal endothelium in primary culture was evaluated and compared with the Na/K ATPase activity that occoured during wounding and healing. Morphologie and ultrastructural changes were investigated by light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The endothelial injury and recovery after blast can be described as a three—stage process. Stage one was characterized by a desquamation, degeneration and necrosis of endothelial cells and a low activity of Na/K ATPase; In stage two, some surviving endothelial cells appeared to migrate and divide and the activity of Na/K ATPase began to increase. In stage three, surviving endothelial cells displayed a flattened configuration consisting of polygons and a high ATPase activity.