失血性休克致大鼠肠缺血性损伤DAO的变化

Alteration of Diamine Oxidase levels in Intestinal Ischemia from Hemorrhagic Shock in Rats

通过测定大鼠失血性休克后肠及血浆二胺氧化酶(DAO),评价DAO在急性肠缺血诊断中的意义。结果表明,休克复苏后6小时降至最低(对照组的36.5%),复苏后24小时有显著回升。血浆DAO改变与肠DAO改变呈正相关(r=0.857,P<0.05)。二者改变与肠粘膜损伤程度相一致。测定DAO可作为早期急性肠缺血诊断的有价值的参考指标。

To evaluate the significance of diamine oxidase (DAO) in the diagnosis of intestinal ischemia, we measured DAO in intestinal tissue and plasma of gnotobiotic rats after hemorrhagic shock. The results showed that intestinal DAO decreased significantly (74.6% of contral value) 2h after resuscitation, 36.5% of contral value at 6h, and then rose obviously at 24 h. There was a positive correlation between DAO in plasma and intestinal tissue (r=0. 857, p<0. 05). Decreased DAO in plasma activity correlated with the extent of histologic injury of the intestinal mucosa. This biochemical marker might provide a valuable tool for the early diagnosis of intestinal ischemia.