自制纤维蛋白胶治疗抗凝兔局部肝创伤

Self—Made Fibrin Glue to treat Local Liver Trauma of Anticoagulated Rabbit

作者旨在探索自制纤维蛋白胶的方法及其在抗凝兔肝创伤模型上的局部止血方法、疗效和组织反应,为临床应用提供实验依据。采用双冷冻—解冻—离心沉淀法从新鲜冷冻血浆中提取浓缩纤维蛋白原,并与猪凝血酶结合制成纤维蛋白胶,应用在抗凝兔的不同类型的肝创面上,进行大体、光镜和电镜观察。结果显示:自制纤维蛋白胶在肝素化肝创伤模型上有较好的止血作用,无严重组织反应和细胞毒性,可满足肝创面止血的需要,并具有制备方法简便、使用安全、价廉等优点,是商业纤维蛋白胶理想的替代品。

This experiment aims at exploring the prepared method of self—made fibrin glue (SMFG), its loeal hemostatic ways, curative effects and tissue reactions in the model of anticoagulated rabbits, to offer a experimental basis for clinical application. SMFG was made by a combination of swine thrombin and pyknotic fibrinogen extracted from fresh frozen plasma through double freezing, unfreezing, centrifugation—precipitation. Using SMFG in various liver wounds, local wounds were observed by gross, optical microscopic and electron microscopic ways. The results showed that SMFG had good hemostatic capacity in the model of heparinized liver trauma, without serious tissue reactions and cellular toxicity. It could satisfy the need of hemostasis in liver trauma, at the same time, it possesses some superiority of simple, safety and cheapness, and could be the best ideal alterative of commercial fibrin glue.