葡萄糖转运蛋白基因多态与中国人NIDDM的关系

THE RELATIONSHIP BETWEEN GLUT/Bgl Ⅱ,Xba Ⅰ RFLP AND CHINESE NON-INSULIN-DEPENDENT DIABETES MELLITUS

基因组DNA以BglⅡ、XbaⅠ限制性内切酶消化,用phGT_(22)为探针杂交,分别得到7.8kb(B_1)、6.2kb(B_2)片断和6.3kb(X_1)、6.0kb(X_2)片断。上海中国人等位基因频率NIDDM组B1、B2为89％和11％,对照组B1、B2为90％和10％,旧金山中国人NIDDM B1、B2为91％和9％,X1、X2为24％和76％,对照组B1、B2为93％和7％,X1、X2为20％和80％。上海中国人与旧金山中国人NIDDM与对照组全组及体重指数亚组差异均无显著性,同样AC、TG亚组间差异亦无显著性。在人种差异分析中,BglⅢ/GLUT色人与黑人组、中国人与黑人组差异均有显著性(x^2=10.07,P<0.01;x^2=9.83,P<0.01)。

Digestion of human genomic DNA with restriction enzyme Bgl Ⅱ, Xba I revealed a two allele polymorphism with a human HepG 2 glucose transporter probe. Bands of 7.8 Kb (Bl allele), 6.2 Kb (B2 allele) and 6.3 Kb (Ⅺ allele), 6.0 Kb (X2 allele) were observed. The genotype frequencies were investigated in two groups of Shanghai Chinese and San Francisco Chinese and their subgroups. The frequencies of BIBI, B1B2 and B2B2 were 82%, 17% 1% respectively among 92 Shanghai Chinese controls, and 78%, 21%, 1 % respectively among 156 NIDDM subjects; 85%, 15%, 0% respectively among 68 San Francisco controls, and 83% 16%, 1% respectively among 90 NIDDM subjects. Most of alleles of Bgl II digestion were allele 1. No significant difference was found between NIDDM and control groups and between BMI<24 and ≥24 subgroups. It is the same in the study of the genotype frequency in subgroups of high triglyceride.