北京地区汉族人补体C4基因的限制性片段长度多态现象

RESTRICTION FRAGMENT LENGTH POLYMORPHISM OF COMPLEMENT C4 GENE IN CHINESE

以Cd基因5'cDNA片段为探针,经DNA印迹杂交法对北京地区60例正常无关汉族个体进行了C4基因限制性片段长度多态性(RFLP)分析。以限制性内切酶HindⅢ消化基因组DNA时检测到5种限制性片段长度变型(RFLV),它们的组成及出现频率为,A:32-25-15 kb(0.433)、B:32-15kb(0.383)、C:25-15 kb(0.117)、D:32-15-8.5 kb(0.05)及E:25-15-8.5(0.017)。此外,还对表现C4A基因缺失的部分样本及数例无C4A基因缺失样本以限制性内切酶BamHⅠ消化其基因组DNA,检出3种RFLV,A:4.5-3.3kb、B:4.5kb及C:3.3kb。

We have applied the technique of Southern blot analysis using a 5' C4 cDNA probe to examine the C_4 gene restriction fragment length polymorphism (RFLP) in Chinese. Hind Ⅲdigestion of genomic DNA in 60 healthy Chinese persons revealed 5 restriction fragment length variants (RFLVs) A: 32-25-15kb, B: 32-15kb, C: 25-15 kb, D: 32-15-8.5 kb and E: 25-15-8.5 kb. The frequencies of these RFLVs were A: 0.433, B: 0.383,C:0.117, D: 0.050 and E: 0.017, respectively. Bam HI digestion in a few samples (including C_4A gene deletion) revealed 3 RFLVs, A: 4.5-3.3 kb, B: 4.5 kb and C:3.3 kb.