温肾助阳药对甲低阳虚模型兔肝组织超微结构的影响

Effect of Decoction for Warming the Kidney and Restoring Yang on Hepatic Ultrastructure of Rabbit Model with Yang Deficiency

应用完全切除家兔甲状腺的手术方法制造阳虚动物模型,并以中药温肾助阳复方进行治疗。应用电子显微镜观察治疗前后肝细胞超微结构的变化。结果显示;甲低阳虚模型兔的肝细胞,其细胞器减少,线粒体减少变性及分布不均,胞质中有大量糖原颗粒,细胞间隙增宽。经中药温肾助阳复方治疗后的肝细胞超微结构与正常对照组相似,线粒体增多,分布较均匀,形态接近正常,糖原颗粒与正常对照组相近。本实验为阳虚动物造型及温肾助阳药治疗阳虚证的疗效提供了有意义的形态学依据。

A rabbit model of Yang deficiency was established by means of total thyrodectomy and the effect of Decoction for warming kidney and restoring Yang (DWKRY) on the hepatic ultrastructure of the model was observed under transmission eletron microscopy. It was shown in the liver cell of the rabbit model that the number of organelles was decreased, the mitochondria were reduced, degenerated and irregularly distributed, a large number of glycogen granules was stored in the cytoplasm, and the intercellular space was widened. After the administtration of DWKRY, the ultrastructure of liver cells approached normal. This study provides a valuable morphological basis for the establishment of Yang-deficiency animal model and the evaluation of the effect of DWKRY on Yang-deficiency syndrome.