常见肿瘤中抗癌基因P53异常的研究

Detection of P53 Gene Abnormalities in Human Tumors

本文研究了部分常见肿瘤组织中P53基因的异常。方法:肿瘤组织DNA经HindⅢ酶切,Southern转移,然后与P53基因探针杂交。结果发现结肠癌中有5/8例发生结构改变(62.5％);胃癌中则有3/9例(33.3％);乳腺癌中有2/15例(13.3％),主要为基因重排或杂合子丢失。这表明P53基因是我国常见肿瘤中基因异常的一个频发位点。

The P53 gene has been implicated as a tumor suppressor gene with mutationsfound in human tumors. By using RFLP analysis in present study, the abnormali-ties of P53 gene were found in 2/15 (13.3%) breast cancer, 3/9 (33.3%) gastriccancer and 5/8 (62.5%) colorectal cancer specimens, but not in the correspond-ing normal tissue DNA. These results indicated that inactivation of P53 gene mayin some cases play an important role in the pathogenesis of human tumors.