手指全血微量ELISA法检测乙型肝炎病毒六项标志物的方法学评价

EVALUATION ON THE METROLOGY OF IMPROVED ASSAY METHOD FOR 6 MARKS ON HBV USING FINGER'S BLOOD AND MICRODETERMINATION ELlSA

本文建立了手指全血微量ELISA法检测乙型肝炎病毒六项标记物的改良方法,并按照卫生部对乙肝标志物检测质控的要求对该方法作了系统的方法学评价,用该法和静脉血清常规法同时检测了240例乙肝六项标志物,两方法检测结果经配对资料显著性检验无显著性差异(P>0.05)。该方法突出的优点是用血量甚微,直接用全血加样,实验所用试剂。

In this paper, we reported the improved assay method for 6 marks on HBV using finger's blood and microdetermination ELISA. It was evaluated with me-thology according to the quality control standard published by the Health Ministry. Two methods were compared using determination of serum of venous blood and using mirrodetermination of finger's blood for dsctetion of 6 marks on HBV in 240 patients. Their identical ratioes were 93.8-98.3%and this method is better than others. It needs only 80ul finger's blood and it doesn't need serum separated from venous blood. During the experiment, only a half dose reagent and sample were used