新生猪胰岛分离及体外培养研究

INVESTIGATIONS ON ISOLATION-AND CULTURE IN VITRO OF NEWBORN PORCINE PANCREATIC ISLETS

本文介绍一种以国产胶原酶消化与组织培养获得新生猪胰岛样细胞团(ICC)的方法。每个新生猪胰腺经浓度为6mg/ml胶原酶消化7—9min,再体外培养8天后,可获取2000～3000ICC。同时,新生猪ICC内β细胞不但快速或慢速相胰岛素分泌功能良好,而且其超微结构亦正常。

A method for the in vitro production of newborn porcine pancreatic islellike cell clusters( ICC) by collagenase digestion and tissue culture was introduced in this paper. 2000-3000 ICC may be isolated from each newborn porcine panrreas which was digested by 6mg/ml collagenase concentration for 7-9 min and cultured for 8 days. Meanwhile, the functions both acute and chronic phase insulin secretion from the cells of the newborn porcine ICC were normal, also the fine structure of them ware excellent. It has been demonstrated that the newborn porcine ICC may be as a potential source both the studies in islet cell growth, and differentiation and xenograft transplantation ic treatment of diabetes