应用AgNOR技术鉴别活检组织高分化鳞癌与鳞状上皮瘤样增生定量研究

QUANTITATIVE STUDY ON AgNOR IN BIOPSY TISSUES OF WELL-DIFFERENTIATED SQUAMOUS CELL CARCINOMA AND TUMOR-LIKE HYPERPLASIA OF SQUAMOUS EPITHELIUM

本文应用AgNOR技术对31例组织形态学上不能确定其性质的活检组织石蜡切片进行AgNOR定量计数分析.结果发现:鳞癌组AgNOR平均值(X)为4.10,标准差(S)为0.51;而增生组(?)为2.68,,S为0.62,经统计学处理t=6.89.P<0.001,表明两组计数具有极显著差异.提示AgNOR染色技术对病变良恶性的鉴别具有一定的辅助诊断价值.同时讨论了在这类病变中AgNOR的计数方法以及两组计数有重叠的原因.

Argyrophilic staining of nucleolar organizer regions (AgNOR) was investigated on routine paraffin-em-bedded histological sections of 14 cases of well-differentiated squamous cell carcinoma and 17 cases of tumor-like hyperplasia of squamous epithelium in which these two lesions couldn't be confirmed by histological methods . The means of AgNOR counts in the two groups of lesions were siguificant (4. 10 with a S of 0. 51 for carcinoma group and 2. 68 with a S of 0. 62 for hyperplasia group t = 6. 89,p<0. 001). The counting methods and the reason of overlapped counts of these two groups of lesions were also discussed. The conclu-sion is that a quantitative assay of AgNOR may be useful to recognize these two lesions.