应用MAC-ELISA方法检测呼吸道合胞病毒IgM抗体

RAPID DIAGNOSIS FOR RESPIRATORY SYNCYTIAL VIRUS INFECTION:APPLICATION OF IgM ANTIBODY CAPTURE ASSAY

建立酶联免疫吸附试验(MAC-ELISA)检测呼吸道合胞(RS)病毒特异性IgM抗体方法,并与中和试验(NT)进行对比。 取35例患毛细支气管炎婴幼儿的急性期及恢复期双份血清共70份标本进行研究。MAC-ELISA测得急性期血清中RS病毒特异性IgM抗体的阳性率为27/35(77.14％),而恢复期血清中和抗体呈≥4倍升高者为23/35(65.71％)。凡NT阳性病例,MAC-ELISA也呈阳性;凡MAC-ELISA阴性病例,NT也呈阴性。另4例NT阴性而MAC-ELISA呈阳性。两者阳性符合率为88.57％。 本研究也观察了IgM抗体与年龄的关系。在6个月龄以前,MAC-ELISA的阳性率远远高于NT。 实验说明采用MAC-ELISA检测RS病毒特异性IgM是可靠的快速诊断方法。

A solid-phase IgM antibody capture-ELISA (MAG-ELISA) was set up to test for serum IgM antibody to respiratory syncytial virus (RSV) and was compared with neutralization test (NT).A total of 70 serum samples taking from 35 infants with bronchiolitis were studied. 27/35 (77.14%) were shown to be RS virus specific IgM antibody positive in acute phase serum by MAO-ELISA while 23/35 (65.71%) showed≥4 times rising of the neutralizing antibodies in convalescent serum by NT. All the positive patients proved by NT were also positive by MAC-ELISA; the negative patients proved by MAC-ELISA were also negative by NT. While the remaining four patients with negative NT result showed positive IgM antibody response. MAO-ELISA was 88.57% correspond with NT.The age dependency of IgM antibody response was also demonstrated clearly in our study. The positive percentage of MAC-ELISA was of much higher as compared with NT until 6 month of age in our observation.The MAC-ELISA was shown to be a reliable technique for rapid