蛋白质抗原的Th及B细胞识别位点的微机分析

PREDICTION OF ANTIGENIC EPITOPE RECOGNIZED BY TH CELLS AND B CELLS WITH COMPUTER

本文报道用高级BASIC语言编制的蛋白质抗原顺序的亲水性及两性分析的微机程序从而推断Th和B细胞的识别位点(epitope)。此程序有三套最常用的氨基酸亲水值数据供选择使用,顺序分析的区段长度可有一定选择。分析时只需输入蛋白质抗原的氨基酸顺序,便可得到计算结果,结果的景佳值选择和作图分析都由计算机完成,由此结果可推断蛋白质分子上B细胞和Th细胞所识别的抗原位点,为人工合成疫苗、抗原决定基分析等提供依据。本程序简单,易于掌握,适用于各种微机。本文以溶菌酶分子为例,用此程序对其氨基酸顺序进行了亲水性和两性分析,并将经计算所推断的抗原位点与溶菌酶分子已知的抗原结构作了比较。

A computer program written in BASIC language for analysing hydrophilicity and amphipathicity characteristics of amino acid sequence blocks of protein molecule is described. Three of the most commonly used hydrophilicity scales can be selected and the block length can also be varied. The operation requires only the insertion of amino acid sequence of a protein and no other information is needed. The peaks of results and graphic analysis are presented in the printout from which we can deduce the antigenic sites of the protein molecule (epitope) recognized by Th cells and B cells. The program is simple, ready-to-use and can be run on any computer. The program output is exemplified by the hydrophilicity and amphipathicity analysis of lysozyme.