摘要
应用抗总的谷胱甘肽转移酶(EC 2.5.1.18 GSTs)的抗体,建立了生物素-亲和素-酶联免疫吸附法(BA-ELISA),并用以检测肿瘤病人血清中总GSTs浓度.本法非常敏感,其最低检出浓度可达0.05ng/ml,在0.05～6.0ng/ml的范围内,血清中总GSTs浓度和其相应之光密度值呈很好的直线关系.批内和批间的变异系数分别为8.2％和12.0％.肝癌、肝炎、消化道肿瘤、良性疾病患者血清总GSTs浓度分别为3.29±2.64,0.83±0.71,0.57±0.47和0.58±0.51ng/ml(mean±SD),而正常对照组仅为0.33±0.29ng/ml.肝癌病人血清总GSTs浓度显著高于其他疾病患者和正常人,总GSTs可望成为肝癌诊断的一个肿瘤标志。
A Biotin-Avidin Enzyme-Linked Immunosorbent Assay (BA-ELISA) for glutath-ione S-transferases (GSTs, 2.5.1.18) in serum was developed. This method is very sensitive, the lowest detection limit is 0.05 ng/ml. The range of the measurement is between 0.05～6.0 ng/ml. The coefficients of the variation of intra-assay and inter-assay were 8.25 and 12.0%, respectively. Several kinds of blood samples got from the patients with cancer and other diseases were investigated in our laboratory using this method. Significant increase of glutathione S-transferases concentration in the serum of the patients with liver cancer was observed indicating that it might be used in the diagnosis of the patients with cancer, especially liver cancer.
出处
《上海免疫学杂志》
CSCD
北大核心
1989年第5期294-296,共3页
Shanghai Journal of Immunology
