小鼠致瘤性CD_4^-CD_8^-早期T细胞在活化胞内双信号条件下可分泌IFN_γ

IFNγ PRODUCTION BY TUMOURIGENIC CD_4^- CD_8^- EARLY T CELLS UNDER THE ACTIVATION OF INTRACELLULAR TRANSDUCTION SIGNAL

本室所建小鼠C_2早期T细胞株,是由内源性逆转录病毒转化的Thy-1^+CD_4^-CD_3^-的致瘤性不成熟T细胞。C_2细胞不能自发地分泌IFN_γ.以PMA+CaI(Ionomycin)活化胞内双信号,C_2细胞可分泌中等度活性的1FN_γ,其分泌能力比正常鼠胸腺皮质细胞群高8倍,但比胸腺髓质细胞群低约3倍.C_2细胞分泌IFN_γ较高可能与逆转录病毒转化相关,而早期T细胞分泌IFN_γ的证实,提示此类细胞可通过其分泌的因子诱导其自身的分化。

The capability of IFNγ production by a murine early T cell line, developed in our lab., referred as C2 cells, has been investigated. C2 cells were endogeneous retrovi-rustransformed tumourigenic cells with phenotype of Thy-1+CD4-CD8-, which could not autonomously produce IFNγ. Under the activation of intracellular transduction signal by PMA + ionomycin, C2 cells could produce IFNγwith the activity of 160u/ml, which was 8 times higher than that produced by cortical type thymocytes of normal mice and approximately 3 times lower than that produced by medullary-type thymocytes. The moderate activity of IFNγ produced by C2 cells might be relevant to the retr-rovirus transformation. The evidence that early T cells can produce IFNγ, however, suggests that CD4-CD8- early T cells may be able to induce their differentiation through their autocrine secretion of IFNγ.