摘要
在免疫印渍技术(Immunoblotting,western blotting)分析斯氏肺吸虫成虫抗原成分,显示22、24、26KD抗原蛋白对该病具诊断价值的基础上,将免疫印渍技术诊断16例斯氏肺吸虫病人,结果全呈阳性反应;同时检测的24例对照血清中、10例日本血吸虫病人、4例华支睾吸虫病人、10例正常人均呈阳性反应.表明22、24、26KD抗原蛋白为斯氏肺吸虫的特异性诊断抗原,免疫印渍技术为斯氏肺吸虫病的高度特异、敏感的一种新型诊断方法。
We had analysed the adult worm antigen of paragonimus skrjnbini(p. s) by immunoblotting and showed that the major protein bands of 22,24,26kD might iave diagnotic value. In present paper this technigue was applied ciinially for fhe diagmosis of paragonimiasis. These three diagnostic bands showed positive reaction with pooled human serumn in all 16 parogommiasis patients.) When 24 control sera, including 10 schistosomiasis. 4 clonochiasis and 10 healthy persons, were tested in the same way. All were negative. The results indicated that 22,24,26kD antigenic proteins of P. s are useful diagnostic antigens and immunoblotting will be a new specific serodiagnostic method for paragonimiasis.
出处
《泸州医学院学报》
1993年第2期97-99,共3页
Journal of Luzhou Medical College
关键词
抗原
免疫诊断
并殖吸虫病
Paragonimiasis
Antigens
Immunediagnosis
Immunoblotting
