短葶山麦冬皂甙C对迟发型变态反应及炎症反应的影响

Effects of Ruscogenin Fucopyranoside on the Delayed Type Hypersensitivity and Inflammatory Reactions

短葶山麦冬皂甙C(Rusco)在抗原激发迟发型变态反应(DTH)前或后ip均能显著地抑制2,4,6-三硝基氯苯(PC)所致的小鼠接触性皮炎,对二甲苯及巴豆油所致的小鼠耳壳炎症反应也有显著的抑制作用。与氢化泼尼松抑制小鼠体重及胸腺、脾脏、肾上腺重量的作用相比,Rusco不影响体重,高剂量时有一定的抑制胸腺的趋势,却能明显增加脾脏和肾上腺重量。上述结果表明,Rusco具有较强的抗炎免疫药理活性,其对迟发型变态反应的抑制作用主要包括抑制T效应细胞的形成及抑制炎症反应等。

A remarkable inhibition of picryl chloride(PC)-induced contact dermatitis(PC-DTH) was observed in mice by the ip injection of ruscogenin 1-O-[β-D-glucopyranosyl (1→2)][β-D-xylopyranosyl (1→3)]-β-D-fucopyranoside (Rusco) either before or after the antigen challenge to elicit DTH. Rusco also markedly inhibited both inflammatory reactions induced by xylene and croton oil in the ear of mice. However, Ruseo did not affect the body weight of mice, and at the higher dose the compound showed a tendency to inhibit thymus, but significantly increased the weights of spleen and adrenal, as compared with prednisolone which strongly decreased all the weights of body, thymus, spleen and adrenal of mice. These results suggest that Rusco possesses strong anti-inflammatory and immunopharmacological activities, and the suppression to delayed type hypersensitivity is displayed mainly through affecting the formation of effector T cells and the inflammatory reaction.