HPLC法分离测定蟾酥脂质体中三种蟾毒内酯成分

Determination of Three Bufadienolides in the Liposome-Ch'an Su by RP-HPLC

脂质体(Liposome)作为药物载体可提高药物的疗效和降低其毒付作用。近年来脂质体药物的研究越来越受到人们重视。

A RP-HPLC method was developed to separate and determine resibufogenin, cinobufagin and bufalin in the liposome-Ch'an su. A ZORBAX ODS column (250×4.6 mm,5 μm) at the room temperature and a mobile phase of acetonitrile-water (53: 47) was used. Flow rate was 1 ml/min, and UV wavelength was 295 nm. The recovery of bufadienolide was 98.4～100.6%, CV<2.58%. The method is simple and rapid.