胎儿垂体细胞离体培养的研究

A Study on the Culture of Pituitary Cells

本文采用机械法制备胎儿垂体细胞悬液后进行了冻存前、后的离体培养。经生长激素(GH)测定、细胞染色及计算机图像分析,证明胎儿垂体细胞冻存前、后经体外培养均有活性。本方法成本低廉、简便易行。为垂体的适时移植增加了供体来来。

Human fetal pituitary cell suspensions prepared with rhechanical method were cultured in vitro before and after being cryopreserved in liquid nitrogen. The results of growth hormone(GH)determination, cell stain and computer image analysis showed that cells of both fresh and cryopreserved pituitary were of biological viability. This method is simple and costs little, It provides another donour source for pituitary transplantation.