在线检测用发酵液透析分离器的研究

A Dialysis-seperation Cell for In-line Determination of Glucose in Fermentation Broth

设计了一种膜透析分离器,以期用于发酵液中葡萄糖浓度的在线检测。对两种膜材料进行了比较,研究了压差、流速、温度和载流液种类对透析分离的影响。在给定的条件下,葡萄糖浓度在10—70mmol/L范围内透过率约为12%。透过率随操作压差、温度而变化,重现性好,CV<4%,对酵母发酵液进行透析分离,葡萄糖透过率至少稳定48h。用于发酵过程在线检测,结果与离线检测相吻合,相关系数r=0.985。

A thin channel dialysis-seperation cell was designed and tested for its use in in-line determination of glucose in fermentation broth, The glucose contained in effluents of both sample stream channel (Channel A) and carrier stream channel (Channel B) was detected with an enzyme electrode flow injection analysis system. Glucose penetration rate was evaluated and defined as R, R = G_b/(G_a+G_b), here R was glucose penetration rate under certain condition, G_a and G_b meant glucose concentration in effluents of channel A and B respectively. Higher penetration rate was obtained when using phosphate buffer (0.01mol/L) as carrier solution instead of using distilled water. Operation pressure difference, temperature and residential time affected glucose penetration. Under the conditon of 0.02MPa pressure differences and 0.23 minute of residential time, the glucose penetration keeped at about 12% in range of 10—70mmol/L and showed good reproducibility with CV<4%. When sample stream was yeast broth, the glucose penetration rate was stable for 48 hours at least. The cell had been used for in-line determination of glucose in a yeast fermentation process, good relationship was observed between in-line and off-line determination of glucose, the corelation coefficient, r=0.985.