正常人与子宫内膜异位症患者子宫内膜抗原的研究

A study of endometrial antigens in fertile controls and patients with endometriosis

对正常人和子宫内膜异位症(EMT)患者的子宫内膜抗原(EMAg)进行了分析。Western blot试验显示,与 EMT 患者血清抗子宫内膜抗体(EMAb)反应的正常人 EMAg 有25、36、46KD 区带,EMT 患者的 EMAg 有36、48、82、94、110、120和140KD 区带。以 EMT 的 EMAg 作为包被抗原,用 ELISA 法检测血清 EMAb 的阳性率高于用正常人 EMAg 包被组(P<0.01)。两种 EMAg 中糖原、氨基酸和元素水平亦有差异。提示 EMAg 成分的变化可能与 EMT 不孕机制相关。

Endometrial antigens (EMAg) in endometrium of fertile controls and patients withendometriosis were analysed.It showed that bounds with MW 25,36 and 46 KD in EMAg from fertilecontrols and that with MW 36,48,82,94,110,120 and 140 KD in EMAg with endometriosis were di-rected against serum endometrial antibodies (EMAb) with endometriosis by Western blot analysis.Weused ELISA assay to detect the positivity of serum EMAb,in which the coat antigens was from patientswith endometriosis,and found that it was significantly higher than fertile controls (P<0.01).There weredifferences in levels of glycogen,amino acids and microelements in EMAg between fertile controls andpatients with endometriosis.It is suggested that changes of EMAg components may lead to the under-standing of the mechanism of infertility associated with endometriosis.