肿瘤坏死因子及前列腺素对卵巢颗粒细胞增殖及雌、孕激素分泌的影响

The influence tumor necrosis factor and prostaglandin upon granulosa cell proliferation and progesterone and estradiol secretion

应用来自体外授精(IVF)或配子移植(GIFT)患者的卵巢颗粒黄体细胞(GL细胞),纯化后培养10日,观察在肿瘤坏死因子α(TNF_α)作用下,对细胞增殖及雌、孕激素分泌的影响。加入重组人TNF_α0.1～10ng/ml后,GL细胞增殖加快,以第0～2日最显著,TNF_α10ng组较对照组增殖一倍。在培养第4～10日TNF10ng组孕激素(P_4)和雌激素(E_2)分泌量增加。若按细胞计每细胞P_4分泌量并无增加而E_2在第8～10日分泌量增加,在培养的GL细胞中加入前列腺素E_2(PGE_2)时,显示PGE_2抑制TNF使GL细胞增殖的作用。提示TNF可促进GL细胞增殖及P_4、E_2分泌增加,并对黄体形成起一定作用。

Human ovarian granuloa luteal cells obtained from an in vitro fertilization program were purified by percoll gradiants and cultured for up to 10 days. Recombinant human tumor necrosis factor alpha (TNF_α)at doses of 0.1~ 10.0ng/ml increased cell number. TNF_α at 10ng/ml doubled cell number on the tenth day of culture when compared to controls. In response to TNF_α 10ng/ml, the fastest growth period was during the first 2 days of culture,as compared to that during days 2～10 TNF_α only at 10ng/ml increased progesterone accumulation in the media during days 4～10 of the culture period. When the amount of progesterone was expressed in ng per 1000 cells,there was no significant difference between TNF-treated cells and the controls. TNF_α also stimulated the production of estradiol on days 2～10 of culture. When expressed as per 1000 cells, estradiol concentrations in the 10ng TNF group were higher than controls on day 8～10. Addition of PGE_2(10mol/L)to culture media prevented the TNF-induced granulosa cells proliferation. These results show that TNF_α promotes cellular events associated with formation of the corpus luteum;i. e. granulosa cell proliferation and steroidogenesis.