牙龈卟啉菌荚膜的提取、纯化

Extraction and purification of capaular material from porphyroaonas gingivalia.

以盐析和高效液相色谱提取、纯化了牙龈卟啉菌荚膜。透射电镜证实提取物来自荚膜,盐析粗提率为1.12％,纯化荚膜占细菌干重的0.22％,其主要成份为糖蛋白,其中糖和蛋白质的比例为1:8.9。细胞毒性检测显示纯化的牙龈叶啉菌荚膜对成纤维细胞L929具有明显的细胞毒性。

Capsular material from P. gingivalis was extracted with saline and purified by HPLC. Transmission electron microscopy elimination revealed that extracted material came from capsule. Crude extraction rate was 1.12%, purified capsular material made up 0.22% of lyophilized P.gingivalis.Preliminary chemical analysis indicated it may be a glyrcoprotein, carbohydrate: prote in ratio was 1 : 8.8. Cell toxicity measure revealed that purified capsular material possessed significant effect on fibroblast L929.