伤寒杆菌L型抗原性及致病性的研究

STUDIES ON THE ANTIGENICITY AND PATHOGENICITY OF SALMONELLA TYPHOSA L FORMS

本文用伤寒杆菌L型和细菌型分别免疫家兔,所得免疫血清经试管凝集法证明:L型的抗O及抗H抗体的效价远较细菌型为低(P<0.01、P<0.001),给小鼠口服感染伤寒杆菌L型及其细菌型(2×10~7个菌),20、30天后处死小鼠进行组织学检查,两组均出现多脏器器间质性炎症,但L型致病性较细菌型为弱。用免疫过氧化物酶技术(PAP法)检测出感染小鼠肠组织切片中有相应抗原存在。实验证明L型抗原在体内存在的时间较长。

In this article, rabbits were immunized with L forms and vegetative forms of S. typhi and the immune sera obtained were examined with the agglutination test. The titers of an-ti- L forms sera were much lower than those of anti vegetative forms when tested with O and H antigen (P<0.01, P<0.001). Mice were infected orally with L forms and vegetative forms of S. typhi separately and sacrificed 20,30 days after infection of pathogens and histological examination. Neither vegetative nor L forms were isolated from blood, bile, mesentery lymph nodes and kidneys. In histological examination, interstitial inflammation in lungs, livers and spleens were seen in both groups. The pathological changes were much severe in the animals infected with vegetative forms with edema and haemorrhage in lungs and massive necrosis In livers and spleens. Since these infected animals were negative in isolation, pcroxidasc antipcroxidasc test was employed to examine the antigens left in tissues. L form antigens were demonstrated in small intestines of animals infected with L forms(7/7) and vcgctativc( 10/12),whereas bacterial antigcncs were demonstrated in 2/7 and 4/12 separately. The persistence of L form antigens appeared to be longer than that of bacterial antigen in tissues. The results suggest that S. typhi L forms arc still pathogenic with animals.