免疫制剂对肝癌患者枯否细胞和单核细胞体外抑制肝癌细胞增殖的影响

IN VITRO CYTOSTATIC OF HUMAN KUPFFERCELLS AND MONOCYTES ACTIVATED BY IMMUNE STIMULANTS ON HUMAN HEPATOMA

取8例人肝癌标本,经分离和培养后获得大量高纯度的枯否细胞(KC)和未贴壁的非肝细胞(NNPCO。从13例肝癌患者血中分离出单核细胞(MC)。以卡介苗(BCG)、短小棒状杆菌(CP)、混合菌苗(MBv)、千扰素(IFN)单用或、2～3种合用,分别处理以上3种效应细胞后,体外检测其对人肝癌细胞SMMC-7721增殖抑制作用的影响。结果显示:①肝癌患者的KC、NNPC和MC对肝癌细胞的增殖仍有明显抑制作用,三者的作用效果相近;②单用免疫制剂中,以BCG提高3种效应细胞的抑瘤作用最明显;③免疫制剂配伍应用较单用效果好,尤以IFN+MBV+BCG作用最强,分别使MC和KG的抑瘤效应提高了125％和101％。本实验为肝癌患者选用免疫制剂提供了依据。

Large numbers of purified Kupffer cells (KC) and nonadherent nonparenohymal cells (NNPC) in surgical samples from eight patients suffering from hepatoma were isolated. Monocytes (MC) were separated from the peripheral blood of thirteen patients. All effector cells were activated in vitro by various immune stimulants, including BCG, Corynebacterium parvum (OP), human white blood cell interferon (IFN) and mixed bacterium vaccine (MBV) respectively. The stimulative effect of two or three stimulants in different combinations were compared with that of single stimulant treatment. The target cell was human hepatoma SMMC-7721. The cytostatio effect of resident and activated effector cells was checked using an in vitro target cell growth inhibition assay, measuring 3H-dT incorporation into tumor cell DNA. Results showed that the patient's three types of effector cells presented similar natural cytostatio properties on hepatoma (41% to 52%). BOC was more effective than any other stimulants in activating effector cells. The stimulative effects of combination stimulants were more effective than that of single stimulant treatment. IPN+MBV+ BOG, which increased oytostasis of MO 125% and KO 101%, was the strongest activator of all the combination stimulant groups.