摘要
用SMMC-7721人肝癌细胞株,经10μmol/L维甲酸(RA)处理后,其生长受到抑制,停留在G0/G1期细胞比例上升,而S、G2+M期细胞的比例下降。光镜及图象分析发现该细胞经RA作用后,形态由长棱形为主且核大转变为以多边形为主且核小,核浆比例由0.595下降至0.247。免疫荧光法检测发现,经RA作用后细胞膜上纤维连接蛋白的含量上升。经RA处理的该株细胞,AFP的分泌量明显低于相应天数的对照细胞,而白蛋白的分泌量则明显增高。结果表明:RA可以从细胞生物学、形态学发生物化学水平逆转人肝癌细胞表型。
The growth rate of SMMC-7721 human hepatocarcinoma cell line was markedly inhibited by the presence of 10 μmol/L retinoio acid (RA, all trans). With flow cytometry, it was found that cells in G0/G1 phase increased while cells in S and G2+M decreased following RA treatment. The morphology of RA-treated cells was changed from fusiform type with large nuclei,to .polygonal type with small nuclei, with 0.695 to 0.247 ratio of nuclei to .plasma. , Fibroneotin on the plasma membrane was significantly increased after RA treatment as determined by immunofluoresoent staining. After RA incubation, simultaneous comparison with control showed decreased secretion of AFP by the , SMMC-7721 cells and enhancement, secretion of albumin. The above results .suggest that RA couldreverse cell biological, morphological and biochemical phenotypes of the human hepatocarcinoma cell in vitro.
出处
《上海医科大学学报》
CSCD
1989年第6期419-422,共4页
Journal of Fudan University(Medical Science)
关键词
肝癌细胞
维甲酸
表型
retinoie acid
hepatocareinoma cell
phenptype