BMP在48例骨及软组织肿瘤的免疫组化研究

Immunohistochemical Study of Bone Morphogenetic Protein in 48 Tumors of Bone and Soft Tissue

以骨形态发生蛋白(BMP)的单克隆抗体ABC法,对36例骨肿瘤、12例软组织肿瘤进行免疫组化染色。结果12例软组织肿瘤阴性(纤维瘤2例,横纹肌肉瘤3例,平滑肌肉瘤7例),骨肿瘤阳性占89%(除外1例骨的恶性纤维组织细胞瘤,1例软骨肉瘤和2例间叶性软骨肉瘤)。认为:①BMP-McAb可用于区分肿瘤是否起源于成骨性结缔组织;②鉴于许多骨肿瘤皆有BMP,但生物学检测只有骨肉瘤、软骨肉瘤和骨样骨瘤有骨生成反应,提出可能和BMP含量有关,并认为免疫组化法优于生物学检测。

Paraffin sections from 12 biopsies of soft tissue tumors and 36 biopsies of bone tumorswere immunostained by ABC method against bone morphogenetic protein(BMP)for investigating thedistribution and diagnostic role of BMP in human bone tumors.The results showed that 12 tumors of ??the soft tissue,including 2 cases of fibroma,3 cases of rhabdomyesarcoma,and 7 cases of leiomyosar-coma were negative.Bone tumors were positive(excepting 1 case of malignant fibrous histocytoma,1case of chondrosarcoma,and 2 cases of mesenchymal chondrosarcoma).It was concluded that:(1)BMP-mcAb may be used to differentiate the tumors,derived from bone-forming cell lines,from theothers,for supplement the morphological criteria which pathologists rley on exclusively for diagnosisbone tumors.(2)a lot of bone tumors contain BMP,but only osteosarcomas,chondros arcomas andosteoid osteomas have been found to induce bone formation by human tumor transplants in athymicnude mouse.It seems possible that inducing bone formation by transplants related not only to existenceof BMP but also to the quantity of BMP which the tumor contained.Immunostaining is better thantransplants.