肝癌DNA含量与生物学特性关系的临床病理学研究

CLINICOPATHOLOGICAL STUDIES ON THE RELATIONSHIP BETWEEN DNA CONTENT AND BIOLOGICAL CHARACTERISTICS OF HEPATOCELLULAR CARCINOMA

本文报道应用图像分析技术,对38例肝癌细胞核DNA含量进行定量测定。结果显示:6例为DNA干系二倍体,32例为DNA干系异倍体。肝癌DNA干系水平与肝癌瘤体大小、包膜突破与癌栓形成发生率以及临床预后等生物学特性有明显相关性,而与临床分期、血清AFP含量、病理分级、组织学类型无关。二倍体肝癌中,66.7％的瘤体直径≤3cm,而异倍体肝癌中,75％的瘤体直径>3cm(P<0.05),提示肝癌于3cm大小时可能是其生物学特性发生转变的重要时期。本文结果表明,对肝癌DNA含量的定量分析,可为临床估价肝癌的生物学特性和判断临床预后提供客观而定量的依据。

DNA content was quantitatively determined by image analysis technique in 38 hepatocellular carcinoma (HCC) cases. The results showed that diploid (2C) DNA stem line was present in 6(15.8％), and aneuploid(AN) DNA stem line in 32(84.2%). DNA stem line of HCC correlated well with the malignant behaviours, such as tumor size, capsule breakage, cancerous thrombus formation and clinical prognosis, but not with the clinical stage, serum AFP content, pathological grade and histological type of HCC. 66.7% of DNA 2C HCC was≤3cm in diameter, whereas 75% of DNA AN HCC was>3cm in diameter (P<0.05),suggesting that when HCC reached 3cm in size,the change of biological characteristics would occur. We consider that the quantitative analysis of DNA content of HCC can provide objective and quantitative basis for predicting the biological features and clinical prognosis of HCC.