新生牛小肠粘膜细胞轮状病毒受体的研制——Ⅰ.新生牛肠粘膜轮状病毒受体抗体的制备

Research on Rotavirus of Receptors of Bovine Small Intestinal Mucosa Cells——Ⅰ.Preparation of Anti-Rotavirus Receptor Antibodies

利用新生牛小肠粘膜细胞为抗原,免疫纯系 Balb/C 鼠。获得鼠抗牛小肠粘膜细胞血清。用提纯的抗 BRV-IgG,建立 ELISA 方法,用以检测以上抗血清。结果表明,抗血清中含有抗 BRV 受体抗体,这种抗体可以在体外模拟 BRV 和兔抗 BRV-IgC 发生特异性结合。该方法可用于抗 BRV 受体单克隆抗体的筛选。

The following methods were used in preparing the anti-rotavirus recedtor anlibo-dies.Cells of new-born bovine upper proportion small intestinal mucosa were used asantigen to immunize the Balb/C mice.The mouse antiserum should include the anti-rotavirus receptor antibodies,as was proved by ELISA.96-well plates were coatedwith anti-bovine rotavirus(BRV)IgG overnight at 4℃,and then the mouse antiserumwas added after incubation overnight at 4℃.The HRP linked rabbit anti-mouse IgGantibody was adbed and incubated for 4h at room temperature.The reaction wasstopped and the substrate O-phenylene diamine was added and finally the OD492 wasread.The anti-BRV receptor antibodies were discovered in the mouse antiserum.Theanti-BRV receptor antibodies might mimic BRV for some applications in vitro.