国人正常胎儿、成人胃粘膜A、B、H、M、N血型抗原的定位及其意义

A,B,H,M,N Blood Group Antigen Location in Chinese Normal Fetal and Adult Gastric Mucosa & Its Significance

首次应用国产单克隆血型抗体,ABC 法,检测国人正常胎儿、成人胃组织石蜡切片 A、B、H、M、N 血型抗原的定位,结合粘液组化染色,判断粘液分泌特点。结果表明:胎儿表面和小凹上皮不完全由粘液细胞组成,血型抗原阳性,分泌混合型粘液;成人胃粘膜血型抗原分为分泌性抗原(A、B、H)和非分泌性抗原(M、N、A、B),粘液中分泌性抗原主要由表面和小凹上皮细胞分泌。提出血型抗原为粘膜屏障的重要组成,为进一步研究胃疾病提供正常形态学和免疫遗传学对照资料。

A,B,H,M,N BGA location was detected in Chinese normal fetal and adult gas-tric mucosae in paraffin sections using home-made monoclonal antibodies by the avidin-biotin-peroxidase complex(ABC)method.The mucus histochemical staining was madein the specimens to decide mucus character.The results showed:The majority of epith-elial cells showed BGA positive and secreting mixed type mucus;BGA of adult gas-tric mucosa might be divided into secretory antigens(A,B,H)and nonsecretory antig-ens(M,N,A,B);The secretory antigen in mucus was secreted by epithelial cells atthe surface and in the pits of the gastric mucosa.It was suggested that blood groupantigen be an improtant composition of the Gastric Mucosal Barrier.This study Pro-vided normal morphological and immunogenesis control material for further researchon gastric diseases.