原发性肝细胞癌患者外周血T细胞亚群、自然杀伤细胞、抗体依赖细胞毒效应改变及其临床意义

PERIPHERAL BLOOD T LYMPHOCYTE SUBSETS, NK, ADCC AND LYMPHOCYTE TRANSFORMATION IN HEPATOCELLULAR CARCINOMA

作者应用单克隆抗体荧光标记流式细胞仪分析系统、微量脱氢酶释放法对121例原发性肝细胞癌(HCC)病人外周血T细胞亚群、自然杀伤细胞(NK)、抗体依赖细胞毒(ADCC)活性、淋巴细胞转化率和花环形成率进行了测定,HCC病人T细胞亚群有明显变化,Th细胞减少和Ts细胞增加NK、ADCC活性和淋转率有程度不同降低。宿主免疫功能变化同病期、肿瘤扩散转移密切相关。Th/Ts比值变化能够敏感反映病人免疫功能状态,是临床观测免疫功能变化的敏感指标。

Using indirect immunofluorescent staining method in micro-blood specimen flow cytometry and micro-LDH releasing test with mouse antihuman T lymphocyte subsets monoclone antibody OKT, the T lymphocyte subsets, NK, ADCC, lymphocyte transformation rate (LT) and rosette formation rate were studied in 121 hepatocellular carcinoma patients, 17 cirrhotics, 12 liver benign tumor patients and 60 healthy persons. In cases with HCC, T lymphocyte subsets were in obvious disorder, including decreased Th subset and increased Ts subset, and dysfunction of NK, ADCC and LT. Th/Ts ratio correlated closely with host cellular immunity, which directly influenced tumor growth, invasion and metastasis. Ts cell would play an important role in immunosuppression of host with HCC. Change of Th/Ts ratio in peripheral blood can sensitively reflect host immunity against HCC and is a valuable immunological criterion.