羊成骨蛋白诱导成骨的电子显微镜观察

AN ELECTRON MICROSCOPIC STUDY OF SHEEP BONE MORPHOGENETIC PROTEIN-INDUCED BONE FORMATION

电镜观察到,sBMP6mg小鼠肌肉内植入后3天,间充质细胞在植入区内聚集并可见由此分化而来的中间型细胞;术后7天,软骨细胞及软骨基质形成;术后14天,软骨细胞变性,骨母细胞及纤维母细胞功能活跃;基质钙化;术后21天,骨母细胞被钙化基质包埋形成骨细胞。sBMP的诱导作用促发了一个完整的骨发育过程,通过软骨内化骨、骨母细胞化骨和纤维母细胞化骨三条途径在局部形成骨组织。文内还讨论了sBMP的靶细胞和诱导成骨过程等问题。

Bone formation induced by sheep bone morphogenetic protein (sBMP) was studied under electron microscope. Three days after implanting 5mg of sBMP in the muscle pouch of rats the osteoprogenitor cells, including prechondro blasts, preosteoblasts and fibroblast-like cells, were differentiated from the induced mesenchymal cells in the implanting area. Seven days later, chondrocytes and cartilage tissue appeared. Fourteen days after implantation, the chondrocytes became denatured, the osteoblasts were located among the cartilaginous tissue and osteoid matrices were formed. By the 21st day, the osteoid matrices were calcified and the osteoblasts were encircled by the calcified matrices and transformed into osteocytes. sBMP evoked a complete process of bone development and new bone formed in the induced area through endochondral ossification and osteoblast osteogenesis and fibroblast osteogenesis.