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免疫荧光技术在临床检验中的应用 被引量:2

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摘要 免疫荧光技术又称荧光素标记技术是指用荧光色素对抗体或抗原进行标记,然后用于免疫学测定。前者称为荧光抗体技术,后者称为荧光抗原技术。在实际工作中主要用荧光抗体技术,荧光抗原技术很少用(用于标记SPA 和亲和素)。荧光抗体技术由Coons 等于1941年建立,当时是用异氰酸荧光黄(fluorescein isocyanatc FIC)来标记抗体,用于在小白鼠的组织切片中检测可溶性肺炎球菌多糖体获得成功。1958年Riggs 等合成了异硫氰酸荧光黄(fluorescein isothiocyanateFITC)性能较FIC 更为优良。
出处 《国外医学(临床生物化学与检验学分册)》 1993年第3期111-113,共3页 Foreign Medical Sciences(section of Clinical Biochemistry and Laboratory Medicine
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