ENNG诱发犬胃癌癌变过程细胞DNA含量变化的研究

STUDY ON CELL DNA CONTENT IN THE CARCINOGENESIS OF CANINE STOMACH INDUCED BY ENNG

本文利用犬胃癌模型,采用Leitz MPV Ⅱ型显微分光光度计,从细胞水平和分子水平,动态地测定细胞核面积和核DNA含量。结果表明,随犬胃粘膜由正常向癌前、肠型癌发展,核DNA含量呈递增性增加(P<0.01),而弥散型癌细胞DNA含量无明显增加。在肠型癌,DNA倍体类型表现为二倍体逐渐减少,多倍体不断增多,而弥散型癌则表现二倍体型。从中度异型增生到高分化腺癌,细胞核面积逐渐增大,癌细胞核面积与核DNA含量呈显著正相关(r=0.73,P<0.01)。提示测定细胞核DNA含量和核面积,可作为诊断肠型胃癌一种新的定量指标,但不适用于弥散型胃癌。

Measurement of nuclear DNA contents and nuclear area by microspectrophotometry was performed in gastric carcinogenesis of three adult wolfdogs induced by ENNG. Nuclear DNA contents were stepwisely increasing and DNA ploid pattern was characterized by the decrease of diploid stemline and the increase of polyploid stemline in the course of development of precancerous and cancerous lesions. But in the cancer of diffuse type, nuclear DNA contents were not changed and ploid pattern was of diploid stemline. From moderate dysplasia to well-differentiated adenocarcinoma, nuclear area was progressively increased. Positive correlation between nuclear DNA contents and nuclear area was presented in cancer cells (r=0.73, p<0.01). These findings indicate that nuclear DNA contents as well as nuclear area could be used as a new quantitative index in the cancer of intestinal type and precancerous lesions, but not in diffuse type.