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酵母核糖核酸的提取及其含量的测定 被引量:5

THE RNA EXTRACTION AND IT'S CONTENT DETERMINATION OF YEAST
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摘要 本文报道了用浓盐法提取利用味精生产中的废水制得饲料酵母中的核糖核酸,并利用721分光光度计对其纯度及含量进行测定,结果表明,核糖核酸的纯度达72%,提取率达6%,若加以治理和综合利用,不但可减少污染,还可变废为利。 This paper introduces the extraction of fee yeat RNA by high salt density methok which was cultivate fromwaste liguicer of monosodium glutamate production. RNA's content and purity wene determined by 721 spectrophotometer. The experiment result inkicates:the RNA purity reae-hes 72% the ektraction rate reades 6%. suppose the waste liquicer be retrieved and used properly enuiroment pollution would be reduced and waste liquicer can be turned into benefit.
出处 《龙岩师专学报》 1993年第3期95-98,共4页 Journal of Longyan Teachers College
关键词 核糖核酸 酵母 酵母核糖核酸 测定 high salt density RNA yeast.
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同被引文献32

  • 1王凤琴.啤酒废酵母泥提取RNA生产工艺研究[J].淮阴工学院学报,1999,8(3):11-15. 被引量:13
  • 2裴杰萍,端青.DNA提取方法的研究进展[J].微生物学免疫学进展,2004,32(3):76-78. 被引量:32
  • 3张帅.氨解法在啤酒酵母中提取RNA的应用与研究[J].广州食品工业科技,2004,20(4):36-37. 被引量:6
  • 4王玉成,张国栋,姜静.一种适用范围广的总RNA提取方法[J].植物研究,2006,26(1):84-87. 被引量:49
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  • 6宋大伟,顾景凯.啤酒废酵母提取RNA工艺研究[D].长春:吉林大学.2005:24-30.
  • 7GAUDREAU H, CONWAY I, CHAMPAGNE C P, Production of zinc enriched yeast extract[J]. Journal of Food Science and Technology, 2001, 38(4): 348-351.
  • 8Zhou J, Brans MA, Tiedje JM. DNA recovery from soils of diverse composition [J]. Appl Environ Microbiol, 1996, 62 (2): 316- 322.
  • 9Loffler J, Hehart H, Schumaeher U, Reitze H, Einsele H. Comparison of Different Methods for Extraction of DNA of Fungal Pathogens from Cultures and Blood [J]. J Clin Mierobiol, 1997, 35 (12): 3311-3312.
  • 10Tsair-Bor Yen, Diana L. Six, Edwin J. Burke. Evaluation of rapid DNA extraction and identification of Phelllnus pini associated wit Pinus eontorta by rDNA assay[J]. Technical Note, 2006, 56 (11/12): 107-110.

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