肝素制剂对人肺巨细胞癌细胞体外粘连性和移动性的影响

EFFECT OF HEPARIN ON THE ADHESION AND MIGRATION OF HUMAN GIANT LUNG CANCER CELLS IN VITRO

观察了肝素钠和肝素钙对人肺巨细胞癌细胞(PLA-801)体外粘连性和移动性,以及对层粘连蛋白促进PLA-801细胞体外粘连性、移动性的影响。结果表明,两种肝素制剂对该细胞的体外粘连性和移动性都显示抑制作用,并能在很大程度上抑制层粘连蛋白对PLA-801细胞体外粘连性和移动性的促进作用。扫描电镜下,经肝素钠和肝素钙处理的细胞丝状伪足减少,这可能是肝素钠和肝素钙抑制该细胞休外粘连性和移动性的形态学基础。此外,层粘连蛋白促进PLA-801细胞铺展的作用,也被肝素钠和肝素钙所抑制。

The effect of heparin-Na and heparin-Ca on the adhesion and migration of PLA-801 human giant lung cancer cells in vitro was invested. It was demonstrated that the adhesion and migration of PLA-801 cells were inhibited by heparin-Na or heparin-Ca. Under scanning electron microscopy, cells treated by heparin-Na or heparin-Ca showed reduction of fdopodia. The reduction of filopodia may be the morphologic basis of decreased adhesion and migration of the cells. Furthermore, heparin-Na and heparin-Ca dispelcd to a great degree the promoting effect of laminin on addhesion, migration and spreading of the cells. The abilities of adhesion and migration of tumor cells, and the promoting effect of laminin on them are regarded as the important factors in tumor metastasis, so it is considered that the inhibition effects of heparin-Na and heparin-Ca on these factors are a novel function of heparin against tumor metastasis.