小鼠腹水支持Sp2/0细胞生长作用的研究

OBSERVATION ON Sp2/0 CELL GROWTH IN CULTURE MEDIUM CONTAINING MOUSE ASCITIC FLUID

不同浓度的腹水及小牛血清RPMI1640培养基对Sp2/0细胞进行培养,结果表明,5％和10％小鼠艾氏腹水的培养基促进Sp2/0细胞增殖作用分别优于10％和15％小牛血清培养基。10％或15％小牛血清培养基中加入1％小鼠艾氏腹水可显示二者具有明显的协同作用。5％小鼠艾氏腹水培养基支持Sp2/0细胞克隆形成率最高,它超过20％小牛血清培养基(小牛血清培养基的最好克隆条件)克隆数的155％。这些结果可能为以Sp2/0细胞作为亲本的杂交瘤细胞提供有意义的生长条件。

The effect ot the medium RPMI-1640 contained 5％ or 10％ mouse ascitic fluid was compared with that of 10％ or 15％ fctal calf serum(FCS) in the Sp2/0 mycloma cell culture in vitro. The Sp2/0 myeloma cells were growing very well in the medium contained the mouse ascitic fluid supported cell growth and clonal formation. It might be useful for the hybridoma technique.