摘要从栽培大豆(Glycine max L)的未成熟子叶游离的原生质体,以Gelrite bead法包埋,培养在大豆根瘤产物(天冬酰胺、谷氨酰胺、尿囊素、尿囊酸等)为主要氮源的ZSP培养基中,形成了胚性愈伤组织。该愈伤组织在体细胞胚分化培养基上直接分化出体细胞胚,并进一步诱导出再生植株,进入正常发育而开花。
1Zhi-ming Wei,Zhi-hong Xu. Plant regeneration from protoplasts of soybean (Glycine max L.)[J] 1988,Plant Cell Reports(5):348～351
2T. Komatsuda,K. Ohyama. Genotypes of high competence for somatic embryogenesis and plant regeneration in soybean Glycine max[J] 1988,Theoretical and Applied Genetics(5):695～700
3J. P. Ranch,L. Oglesby,A. C. Zielinski,R. B. Horsch. Plant regeneration from embryo-derived tissue cultures of soybeans[J] 1985,In Vitro Cellular & Developmental Biology(11):653～658
4C. A. Newell,H. T. Luu. Protoplast culture and plant regeneration in Glycine canescens F.J. Herm[J] 1985,Plant Cell, Tissue and Organ Culture(2):145～149
5K. N. Kao. Chromosomal behaviour in somatic hybrids of soybean-Nicotiana glauca[J] 1977,MGG Molecular & General Genetics(3):225～230
