过硼酸盐作底物滴定法测定红细胞及心肌组织中过氧化氢酶活力

Assay of the activity of catalase in erythrocytes and myocardium in rats by titrimetry with perborate as substrate

本文采用过硼酸钠作底物的高锰酸钾反滴定法测定了红细胞溶血制品及心肌匀浆中的过氧化氢酶活力。随红细胞稀释度的增高,酶活力急剧下降。有机溶剂及表面活性剂不能明显提高心肌匀浆制品的酶活力。测定反应时程如超过2min,测得的酶活力逐渐降低。平行样品测定结果证实此方法具有良好的精确度。此法简单、快速、稳定,适于一般实验室应用。

The activity of catalase in hemolysates of erythrocytes and myocardial homogenate in rats was measured by back titration method with permanganate, using perborate as the substrate. There was a relatively rapid loss of the activity of catalase with dilution of the hemolysates. Addition of organic solvent and surface-active agent did not increase remarkably the enzymic activity in myocardial homogenate. The activity of catalase degraded wehn incubation was over two min. Based on measurement of 10 parallel samples, 2.05 was found to be the coefficient of variation. The present method is simple, rapid, stable and suitable for application in common laboratories.