正常人240个人精子染色体核型分析

THE CHROMOSOME CARYPES OF 240 SPERMATOZOAFROM NORMAL MEN

应用人精子与去透明带金黄地鼠卵受精技术制备人精子染色体标本，对正常男性２４０个精子核型进行了分析。结果显示，数目畸变精子率为３．３４％，结构畸变精子率为６．６７％。所观察到的结构畸变类型为无着丝粒断片，染色体断裂、双着丝粒体、易位和染色单体断裂。在正常男性精子染色体组中，Ｘ一精子与Ｙ一精子的比例为４９．１７％：５０．８３％；近端着丝粒染色体随体联合显随机分布，随体联合频率为０．０９６。对上述实验结果的意义进行了讨论。

With interspecific fertilization in vitro between zona-free hamster oocytes and human spermatozoa,240 human sperm chromosome caryotypes were analyzed. The results showed that the rates of spermatozoa with numerical and structural aberrations were 3.34%,6.67% respectively ,The type of structural chromosome aberrations observed was fragment,break,dicentrics,translocations and chromatid break.The ratio of X-to Y-sperm was 49. 17% to 50.83%.The random distribution of satellite association(SA)of acrocentrics in human sperm chromosome caryotypes was demonstrated,and the frequency of SA was 0.096.Results were discussed.