兴安升麻总皂甙对丝裂霉素C诱发人外周血淋巴细胞SCE频率的影响

THE EFFECT OF CLMICIFUGA DAHURICA SAPONINS ON SCEFREQUENCY INDUCED BY MMC IN PERIPHERAL LYMPHOCYTESoF HUMAN

选择正常健康人为对象，研究兴安升麻总皂甙对丝裂霉素Ｃ（ＭＭｃ）诱发人外周血淋巴细胞姐妹染色单体互换（ｓｉｓｔｅｒＣｈｒｏｍａｔｉｄＥｘｃｈａｎｇｅ，ＳＣＥ）频率的影响。实验分为１０．９４μｇ／ｍｌ、４３．７５μｇ／ｍｌ、１７５．０μｇ／ｍｌ３个升麻剂量组，ＤＭＳＯ溶剂对照组，ＭＭＣ阳性对照组和空白对照组，比较不同浓度兴安升麻剂量组，对ｍｍｃ诱发体外人外周血细胞ＳＣＥ频率的差异。结果表明，ＭＭＣ加兴安升麻组ＳＣＥ频率与对照组比较，有显著统计学意义的改变（Ｐ＜０．００１），提示兴安升麻是个潜在的抗突变剂。

The effect of Cimicifuga dahurica Saponins on SCE frequency induced by MMC in peripheral lymphocytes of healthy human was studied.There were six experimental groups,i.e.10.94μg/ml,43.75μg/ml, 175.0μg/ml Cimicifuga dahurica Saponins,DMSO control group,MMC positive control group and negative control group,compared with each of three control groups,all three Cimicifuga dahurica saponins groups showed dramatic changes(P<0.001),The fact that Cimicifuga dahurica Saponins effectively inhibits the SCE frequency induced by MMC in peripheral lymphocytes of normal human indicated that Cimicifuga dahurica Saponins is a potential antimutagenic agent.