环氧乙烷的遗传毒性研究Ⅱ．小鼠精子畸形试验

STUDY ON THE GENOTOXICITY OF ETHYLENE OXIDEⅡ. SPERM ABNORMALITIES TEST IN MICE

本文用亚急性和亚慢性２种处理方法，观察了（ｅｔｈｙｌｅｎｅｏｘｉｄｅ，Ｅｔｏ）对小鼠精子畸形的诱变率，发现亚急性处理组只有高浓度（５４０ｍｇ／ｍ）第７ｄ取材的与对照组比较有显著的诱变率（１５２±７６．２７Ｐ＜０．０１），且具明显的剂量─效应关系；而大于７ｄ的以及其它各种浓度的处理与对照组相比均无显著差异．但亚慢性处理组连续１４ｗｋ的吸入，其低浓度（５４ｍｇ／ｍ３）与高浓度（３６０ｍｇ／ｍ３）的吸入与对照组比较，都有明显的诱变率（分别为２９．４±ｌ６．２４，Ｐ＜０．０５和５２．９１±１１．９７，Ｐ＜０．００１）。结果表明小剂量长期吸入造成的诱变性明显，也比较符合化工工人长期操作接触化学物剂量的实际悄况，特别对男性工人接触Ｅｔｏ后引起对后代的致畸或不育症的潜在可能性更应予以关注及进一步研究。

The mutation frequencies of sperm abnormalities in mice with subacute and subchronic testswere studied.We found the only significant mutation frequency (152± 76.27) between the high concentra-tion (540mg/m)group and control group of subacute test on the seventh day(P< 0.01 ). And thedose─effect relationship is dominant. There are no significant difference between the other concentrdationgroups and control groups after seven days.But in the subchronic test with the mice inhaling ethylene ox-ide for 14 weeks continuously,low(54mg/m3)and high(360mg/m3)concentration groups were compared with control group and their mutation frequencies are obvious(They are 29.4±16.24,P<0.05 and52.91±11.97,P<0.001,respectively).These results showed that it is evident mutagenesis toinhaleethylene oxide for a long time although in the low concentration.It coincides with the actual situation ofworkers.who are occupationally exposured to chemicals for a long time. We must pay attention to the possibility of teratogenesis in offspring and infertility among male workers potentally exposed to ethyleneoxide.and this subject should be studied further.