膦氧氮丙啶的细胞恶性转化研究

CELL MALIGANNT TRANSFORMATION IN VITRO IN-DUCDE BY TRIS (2-METHYL-1-AZIRIDINYL) PHOSPHINEOXIDE

本试验采用叙利亚金黄地鼠胚胎Ｓｙｒｉａｎｈａｍｓｔｅｒｅｍｂｒｙｏ（ＳＨＥ）细胞的体外恶性转化方法，检测出Ｔｒｉｓ（２－ｍｅｔｈｙｌ－１－ａｚｉｒｉｄｉｎｙｌ）ｐｈｏｓｐｏｈｉｎｅｏｘｉｄｅ（ＭＡＰＯ）可诱导ＳＨＥ细胞形态学恶性转化。转化细胞嗜碱性强、核浆比例增大、排列方向紊乱、可交叉重叠生长。从转化集落分离的细胞生长旺盛、染色体数目异常、可被较低浓度的植物凝集素凝集、在半固体琼脂内非贴壁依赖生长、接种免疫抑制动物可长出肿瘤。上述结果表明：ＭＡＰＯ具有诱导ＳＨＥ细胞恶性转化的作用，预示它对人类有潜在的致癌危险性。

Tris(2-methyl-1-aziridiny 1) phosphine oxidc (MAPO)was able to inducephenotype transformation of syrian hamster embryo (SHE) cells. The transformantsproliferated rapidly, showed increased basophilia, as well as an increase in the ratio of nu-cleus to cytoplasm, and their lifespan extended notably. The transformed colonies exhibitedextensive random orientation and crossing-over of the cells. Chromosome number ofMAPO transformants changed remarkably. The transformed cells could be agglutinated bylower concertnation phytohemagglutinin, and could grow in semisolid agar, produce tu-mors in immunosuppressed newborn syrian hamstcr. These results suggest that MAPO is able to induce malignant transformation of SHEcells, and its potential carcinogenicity to human is concerned.