顺铂对雄性小鼠生殖细胞的遗传毒性

GENOTOXIC EFFECT OF CDOP ON GERM CELL OF MALEMOUSE

无机抗癌剂氯氨顺铂（ＣＤＤＰ）可诱发小鼠初级精母细胞染色体畸变（ＰＳＣＡ）和精子畸形（ＳＡＭ），其诱发率分别在２．５－１５．０ｍｇ／ｋｇ和５．０－２０．０ｍｇ／ｋｇ体重剂量范围内具有剂量依赖关系。ＣＤＤＰ锈发ＰＳＣＡ和ＳＡＭ的最低检出剂量（ＭＤＤ）分别５．３８和５．４０ｍｇ／ｋｇ体重，两者在统计学上无明显差异，但ｌｍＭ／ｋｇ体重的ＣＤＤＰ诱发的ＰＳＣＡ和ＳＡＭ的细胞个数差异很大，前者为３．９６×１０￣１７个，后者为４１５．４２个，这可能是由于ＳＡＭ试验从给药至取样的时间间隔较ＰＳＣＡ试验者长，大量的受损生殖细胞丧失的关系。

Primary spermatocyte chromosome aberration (PSCA)and sperm abnormalmorphology (SAM ) may be induced by cis─diamminedichloroplatinum(Ⅱ) (CDDP)which a inorganic anticarcinogenic agent and the frequencies of PSCA and SAM induced byCDDP, deponded on total dose of the drug in 2.5─15 mg/kg and 5─20 mg/kg body weightin male mice respectively。 Minimum detected dose of CDDP for inducing PSCA and SAMwere 5.38mg/kg and 5. 40 mg/kg body weight respectively. They had no significantdifference in statistics,but the number of cells with PSCA and SAM induced by onemilligram molecle of CDDP/kg body weight had a large defference. The former wastheoretically 3.96×10￣17 and the later was theoretically 41 5.42. The lower value of SAMinduced by CDDP may be due to the longer interval of the SAM test from delivering thedrug to taking speciman than that of the PSCA test and the large number of damaged germcell to be lost.