5──溴脱氧尿嘧啶核苷标记法检测哺乳动物体细胞染色体异常分离的研究

ANALYSYSIS OF CIIROMOSOME MALSEGREGATION IN MAMMALIAN SOMATIC CELLS WITH BRDU LABELLING TECH-NIQUE

通过分析５一溴脱氧尿嘧啶核苷（５－ｂｒｏｍｏｄｅｏｘｙｕｒｉｄｉｎｅ，ＢｒｄＵ）掺入后的第２周期细胞染色体，同步评估了受试化合物秋水仙素（ｃｏｌｃｈｉｃｉｎｅ，ＣＯＬ）及昆明山海棠水抽提物（Ｔｒｉｐｔｅｒｙｇｕｉｍｈｙｐｏｇｌａｕｃｕｍ（Ｌｅｕｌ）Ｈｕｔｃｈ，ＴＨＨ）在小鼠骨髓细胞中的有丝分裂阻滞、染色体异常分离及姐妹染色单体互换（ｓｉｓｔｅｒｃｈｒｏｍａｔｉｄｅｘｃｈａｎｇｅ，ＳＣＥ）诱发效应。ＣＯＬ及ＴＨＨ均具有不同程度的有丝分裂阻滞效应，且显著诱发非整倍体（２ｎ＝４１－４３）及多倍体的产生。ＴＨＨ尚显著诱发小鼠骨髓细胞ＳＣＥ。结果提示：ＴＨＨ具有与ＣＯＬ类似的哺乳动物体细胞非整倍体／多倍体诱发效应；此外，ＴＨＨ尚含有某些基因毒性成分．实验还证实，ＢｒｄＵ标记法检检测哺乳动物体细胞染色体异常分离是可行的。

Abstract The effects of mitotic block , chromosome malsegregation and sister chromatidexchange(SCE)induction were simultaneously investigated、with Bromodeoxyuridine(Br-dU) labeled mouse bone [marrow cells at their second generation after a single i.p. treat-ment(COL ) and Triperygium hypoglaucum (Leul)Hutch(THH). Significant delay ofcell cycle were found after amimals were treated with both COL and THH. COL and THHalso induced aneuploidy significantly(2n = 41～ 43 ) and polypoloidy in second mitosis.Asignificant increased SCE over the control frequency was oherserved after 5g/kg and 10g/kg of THH treatment.The result indicated that THH share the induction effects of aneu-ploidy and polyploidy as COL. Besides this, THH also possess genotoxicity. The approachto estimate in vivo mitotic malsegregation in mammalian somatic cells based Brdu incorpo-ration is vaiid.